Isolation And Identification Of Cellulolytic Fungi And Bacteria From Fish Pond Effluent

ABSTRACT

Cellulases are the group of hydrolytic enzymes and are capable of degrading all types of cellulosic waste materials. Present study focuses on the isolation and screening of cellulolytic fungi and bacteria from the fish pond effluent in MOUAU. In this present study, cellulolytic bacterial strains were screened and isolated. Six isolates were found to be Gram positive. Which includes Bacillus spp, Micrococcus spp and Staphylococcus, Streptococcus and Gram negative bacterial isolates include E. coli, Proteus species, Klebsiella and Pseudomonas spp. While fungal isolates includes Penicillum species, Aspergillus species, Alternaria species yeast, Rhizopus, Trichoderma.

TABLE OF CONTENT

Certification ﾿ i

Dedication ﾿ ii

Acknowledgements ﾿ iii

Table of contents ﾿ iv

List of Tables ﾿ vi

Abstract ﾿ viii ﾿

CHAPTER ONE

1.1. ﾿ INTRODUCTION ﾿ 1

1.2. ﾿ Cellulolytic fungi and bacteria ﾿ 1

1.3. ﾿ Aim of the Study ﾿ 2

1.4. ﾿ Objectives of the study ﾿ 2

CHAPTER TWO

2.1 LITERATURE REVIEW ﾿ 3

2.1.1 Recommendations for Managing Aquaculture Pond Effluents ﾿ 5

2.2 ﾿       Overview of Fungi ﾿ 6

2.2.1.    Water associated fungi (aquatic fungi) ﾿ 6

2.2.2     Fish Pond as fungi habitat ﾿ 8

2.2.3 ﾿ The Cellulolytic fungi                                                                                           8 ﾿

2.3.     Overview of bacteria ﾿ 9

2.3.1   Growth ﾿ 9

2.3.2.   Factors that affect the generation time of bacteria ﾿ 10

2.3.3  Importance of bacteria ﾿ 11

2.3.4. ﾿ The cellulolytic bacteria                                                                                ﾿ 11

CHAPTER THREE

MATERIALS AND METHODS ﾿ 13

3.1 ﾿ Sample Collection ﾿ 13

3.2 ﾿ Sample, and Sampling and Media Preparations ﾿ 13

3.2.1 ﾿ Sample Preparations. ﾿ 13

3.3       preliminary screening of water samples for cellulolytic microorganism ﾿ 14

3.4       Isolation of cellulolytic organisms ﾿ 14

3.5.0    Characterization of isolates                                                                                   15 ﾿

.3.5.1    Characterization of bacteria isolates ﾿ 15

3.5.2    Colony Morphology test ﾿ 15

3.5.3   Microscopic features. ﾿ 15

3.6.1 Biochemical reaction test ﾿ 15

3.6.2   Catalase Test ﾿ 15

3.6.3 ﾿ Oxidase Test ﾿ 16

3.6.4    Coagulase Test ﾿ 16

3.7.     Sugar utilization test ﾿ 16

3.8.    Characterization of fungi ﾿ 16

3.8.1   Identification of isolate ﾿ 17

CHAPTER FOUR

4.1     RESULT ﾿ 18

CHAPTER FIVE

5.1 DISCUSSION ﾿ 24

5.2CONCLUSSION ﾿ 26

5.3 RECOMMENDATION ﾿ 27

REFERENCES ﾿ 28

APPENDIX ﾿ 32