Antibiogram Of Bacteria Isolated From Different Fish Ponds Waste Waters In Umuahia, Abia State

ABSTRACT

Six bacterial genera were isolated, which include; Pseudomonas aeruginosa, Bacillus sp, Micrococcus luteus, Escherichia coli, Proteus sp and Serratia marcasens. The total heterotrophic bacterial count showed that D1 had the highest count of 8.2 x 104 cfu/ml while F had the lowest count of 5.7 x 104 cfu/ml. The total coliform count showed that D1 had the highest coliform count of 6.9 x 104 cfu/ml, while CW1 had the lowest count of 4.4 x 104 cfu/ml. The percentage occurrence revealed that Escherichia coli had the highest frequency occurrence of 7 (28%) while Pseudomonas aeruginosa had the least frequency occurrence of 1 (4%). The antibiotic sensitivity revealed that all of the organisms are sensitive to some of the antibiotics used, the highest inhibitory activity of 22mm was recorded for Escherichia coli, Serratia marcasens, Proteus sp and Micrococcus luteus on Rifampicin, Ciprofloxacin, Gentamycin and Rifampicin respectively.

TABLE OF CONTENTS

Title Page ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgements ﾿ iv

Table of Contents ﾿ v

Lists of Tables ﾿ vii

Abstract ﾿ viii

CHAPTER ONE

1.0 Introduction ﾿ 1

1.1 ﾿ Aims and Objectives  ﾿ 3

1.2 Objectives ﾿ 3

CHAPTER TWO 

2.0 Literature Review  ﾿ 4

2.1 Bacteria associated with Fish Pond ﾿ 8

CHAPTER THREE 

3.0 Materials and Method ﾿ 16

3.1 Sample Collection ﾿ 16

3.2 Media Used ﾿ 16

3.3 Sterilization  ﾿ 17

3.4 Bacterial count ﾿ 17

3.5 Bacterial Isolation ﾿ 17

3.6 Characterization and Identification of the Bacterial Isolates ﾿ 17

3.6.1 Gram Staining ﾿ 18

3.7 Biochemical Characteristics of the Isolates ﾿ 18

3.7.1 Motility Test ﾿ 18

3.7.2 Indole Test ﾿ 19

3.7.3 Citrate Utilization ﾿ 19

3.7.4 Sugar Fermentation ﾿ 19

3.7.5 Oxidase Test ﾿ ﾿ 20

3.7.6 Coagulase Test ﾿ 20

3.7.7 Urease Test ﾿ 20

3.7.8 Methyl Red/ Voges Proskauer Test ﾿ 21

3.8 Antibiotic Sensitivity Testing ﾿ 21

CHAPTER FOUR

4.0  ﾿ Results ﾿ 22 ﾿

CHAPTER FIVE

5.0       Discussion, Conclusion and Recommendation ﾿ 28

5.1 ﾿ Discussion ﾿ 28

5.2 ﾿ Conclusion ﾿ 29

5.3 ﾿ Recommendation ﾿ 29 ﾿

References ﾿ 31 ﾿